| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Clostridium butyricum | TTCAACAGTTCCACCCCTCC | AGGTGCAATTGATTCCGTGAT | 59.52 | 58.27 | 298 | 131 |
100.00%
|
50.00%
|
| Primer 22 | Clostridium butyricum | ATTCAACAGTTCCACCCCTCC | AGGTGCAATTGATTCCGTGAT | 59.92 | 58.27 | 299 | 131 |
100.00%
|
50.00%
|
| Primer 23 | Clostridium butyricum | AACAGTTCCACCCCTCCCTG | AGGTGCAATTGATTCCGTGAT | 61.43 | 58.27 | 295 | 131 |
100.00%
|
50.00%
|
| Primer 24 | Clostridium butyricum | TCTCCAGGAGTAAAGCTTGGA | TAACTACCGCACCAGCTCC | 58.10 | 59.10 | 262 | 130 |
100.00%
|
50.00%
|
| Primer 25 | Clostridium butyricum | TCAACAGTTCCACCCCTCCC | AGGTGCAATTGATTCCGTGAT | 61.42 | 58.27 | 297 | 130 |
100.00%
|
50.00%
|
| Primer 26 | Clostridium butyricum | AGCAAACCTCAATCTAACGCA | CCTCCGGTTGAACCTTGGA | 58.22 | 59.24 | 296 | 128 |
100.00%
|
50.00%
|
| Primer 27 | Clostridium butyricum | AGCAAACCTCAATCTAACGCA | TCCTCCGGTTGAACCTTGG | 58.22 | 59.24 | 297 | 128 |
100.00%
|
50.00%
|
| Primer 28 | Clostridium butyricum | AGCAAACCTCAATCTAACGCA | TGTCCTCCGGTTGAACCTTG | 58.22 | 59.89 | 299 | 128 |
100.00%
|
50.00%
|
| Primer 29 | Clostridium butyricum | AGCAAACCTCAATCTAACGCA | GTCCTCCGGTTGAACCTTGG | 58.22 | 60.61 | 298 | 128 |
100.00%
|
50.00%
|
| Primer 30 | Clostridium butyricum | AGCAAACCTCAATCTAACGCA | TCCTCCGGTTGAACCTTGGA | 58.22 | 60.77 | 297 | 128 |
100.00%
|
50.00%
|